Bioassay

Bioassay:- It is testing of a biological activity like growth response of a substance by employing a living material like plant or plant part. 

Auxin Bioassay:-

> Auxins are one of the major phytohormones essential for plant growth and development. Normally, in plants, auxins are synthesized in the growing apex and then transported to various regions for physiologi­cal activities.

> In plants, auxins are present in small quantities, thus chemical test of plant materials does not always provide desirable results. As such, bioassay methods are often used for detection of auxin in unknown phytohormone extracts. There are several bioassay techniques for auxin: Avena coleoptile curvature test, Root inhibition test, Wheat coleoptile test, etc.

Gibberellin Bioassay:-

The Gibberellins (GAs) are one of the major groups of growth-promoting hormones which play essential roles in the regulation of growth and development of seed plants. By comparing the response to the extract obtained from plants with a standard response curve obtained by treating dwarf peas with a range of known dosages of GA3, one can assay the quantity of GA3 present in the unknown extract.